To
make 0.1M ATP aliquots:
1) Weigh out ~11g ATP
2) Dissolve in ~150ml cold
water in a 250mL beaker w/stir bar.
3) Add solid KOH to pH to
7.0. This is solution A.
4) Dilute 100uL of solution A
into 1 litre of water, and MIX WELL
5) Take spec reading of this
1:10000 dilution at 260nm blanking against water (OD)
6) Pour solution A into a 250mL
measuring cylinder. Measure volume. (V)
7) Calculate final volume
(Final volume = V*OD/0.154) and make up to final volume in measuring cylinder
with cold water.
8) Mix well and pour into
falcon tubes in 10ml alequats and freeze at -20ēC.