Publications


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WH2 and proline-rich domains of WASP-family proteins collaborate to accelerate actin filament elongation.
Bieling P, Hansen SD, Akin O, Li TD, Hayden CC, Fletcher DA, Mullins RD. EMBO J. 2017 Nov 15. pii: e201797039. doi: 10.15252/embj.201797039.
PMID: 29141912

Actin-based protrusions of migrating neutrophils are intrinsically lamellar and facilitate direction changes.
Fritz-Laylin LK, Riel-Mehan M, Chen BC, Lord SJ, Goddard TD, Ferrin TE, Nicholson-Dykstra SM, Higgs H, Johnson GT, Betzig E, Mullins RD.
eLife. 2017 Sep 26;6. pii: e26990. doi: 10.7554/eLife.26990.
PMID: 28948912

Our evolving view of cell motility.
Fritz-Laylin LK, Lord SJ, Mullins RD.
Cell Cycle. 2017 Aug 18:1-2. doi: 10.1080/15384101.2017.1360655. Editorial.
PMID: 28820330

Bacterial Tubulin A/B Exhibit Polarized Growth, Mixed-Polarity Bundling, and Destabilization by GTP Hydrolysis.
Díaz-Celis C, Risca VI, Hurtado F, Polka JK, Hansen SD, Maturana D, Lagos R, Mullins RD, Monasterio O.
J Bacteriol. 2017 Jul 17. pii: JB.00211-17. doi: 10.1128/JB.00211-17.
PMID: 28716960

WASP and SCAR are evolutionarily conserved in actin-filled pseudopod-based motility.
Fritz-Laylin LK, Lord SJ, Mullins RD.
J Cell Biol. 2017 Jun 5;216(6):1673-1688. doi: 10.1083/jcb.201701074.
PMID: 28473602

Actomyosin dynamics drive local membrane component organization in an in vitro active composite layer.
Köster DV, Husain K, Iljazi E, Bhat A, Bieling P, Mullins RD, Rao M, Mayor S.
Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):E1645-54. doi: 10.1073/pnas.1514030113.
PMID: 26929326

Force Feedback Controls Motor Activity and Mechanical Properties of Self-Assembling Branched Actin Networks.
Bieling P, Li TD, Weichsel J, McGorty R, Jreij P, Huang B, Fletcher DA, Mullins RD.
Cell. 2016 Jan 14;164(1-2):115-27. doi: 10.1016/j.cell.2015.11.057.
PMID: 26771487

Comparative analysis of tools for live cell imaging of actin network architecture.
Belin BJ, Goins LM, Mullins RD.
Bioarchitecture. 2015;4(6):189-202. doi: 10.1080/19490992.2014.1047714.
PMID: 26317264

Lamellipodin promotes actin assembly by clustering Ena/VASP proteins and tethering them to actin filaments.
Hansen SD, Mullins RD.
Elife. 2015 Aug 21;4. doi: 10.7554/eLife.06585.
PMID: 26295568

DNA damage induces nuclear actin filament assembly by Formin-2 and Spire-½ that promotes efficient DNA repair.
Belin BJ, Lee T, Mullins RD.
Elife. 2015 Aug 19;4. doi: 10.7554/eLife.07735.
PMID: 26287480

Arp2/3 complex and cofilin modulate binding of tropomyosin to branched actin networks.
Hsiao JY, Goins LM, Petek NA, Mullins RD.
Curr Biol. 2015 Jun 15;25(12):1573-82. doi: 10.1016/j.cub.2015.04.038.
PMID: 26028436

A novel tropomyosin isoform functions at the mitotic spindle and Golgi in Drosophila.
Goins LM, Mullins RD.
Mol Biol Cell. 2015 Jul 1;26(13):2491-504. doi: 10.1091/mbc.E14-12-1619.
PMID: 25971803

Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution.
Chen BC, Legant WR, Wang K, Shao L, Milkie DE, Davidson MW, Janetopoulos C, Wu XS, Hammer JA 3rd, Liu Z, English BP, Mimori-Kiyosue Y, Romero DP, Ritter AT, Lippincott-Schwartz J, Fritz-Laylin L, Mullins RD, Mitchell DM, Bembenek JN, Reymann AC, Böhme R, Grill SW, Wang JT, Seydoux G, Tulu US, Kiehart DP, Betzig E.
Science. 2014 Oct 24;346(6208):1257998. doi: 10.1126/science.1257998.
PMID: 25342811

Design of reversible, cysteine-targeted Michael acceptors guided by kinetic and computational analysis.
Krishnan S, Miller RM, Tian B, Mullins RD, Jacobson MP, Taunton J.
J Am Chem Soc. 2014 Sep 10;136(36):12624-30. doi: 10.1021/ja505194w.
PMID: 25153195

Micropattern-guided assembly of overlapping pairs of dynamic microtubules.
Fourniol FJ, Li TD, Bieling P, Mullins RD, Fletcher DA, Surrey T.
Methods Enzymol. 2014;540:339-60. doi: 10.1016/B978-0-12-397924-7.00019-4.
PMID: 24630116

Bacterial actin-like proteins: purification and characterization of self-assembly properties.
Petek NA, Mullins RD.
Methods Enzymol. 2014;540:19-34. doi: 10.1016/B978-0-12-397924-7.00002-9.
PMID: 24630099

Accessory factors promote AlfA-dependent plasmid segregation by regulating filament nucleation, disassembly, and bundling.
Polka JK, Kollman JM, Mullins RD.
Proc Natl Acad Sci U S A. 2014 Feb 11;111(6):2176-81. doi: 10.1073/pnas.1304127111.
PMID: 24481252

αE-catenin actin-binding domain alters actin filament conformation and regulates binding of nucleation and disassembly factors.
Hansen SD, Kwiatkowski AV, Ouyang CY, Liu H, Pokutta S, Watkins SC, Volkmann N, Hanein D, Weis WI, Mullins RD, Nelson WJ.
Mol Biol Cell. 2013 Dec;24(23):3710-20. doi: 10.1091/mbc.E13-07-0388.
PMID: 24068324

What we talk about when we talk about nuclear actin.
Belin BJ, Mullins RD.
Nucleus. 2013 Jul-Aug;4(4):291-7. doi: 10.4161/nucl.25960. Review.
PMID: 23934079

Cytoplasmic actin: purification and single molecule assembly assays.
Hansen SD, Zuchero JB, Mullins RD.
Methods Mol Biol. 2013;1046:145-70. doi: 10.1007/978-1-62703-538-5_9.
PMID: 23868587

Visualization of actin filaments and monomers in somatic cell nuclei.
Belin BJ, Cimini BA, Blackburn EH, Mullins RD.
Mol Biol Cell. 2013 Apr;24(7):982-94. doi: 10.1091/mbc.E12-09-0685.
PMID: 23447706

Arp2/3 complex ATP hydrolysis promotes lamellipodial actin network disassembly but is dispensable for assembly.
Ingerman E, Hsiao JY, Mullins RD.
J Cell Biol. 2013 Mar 4;200(5):619-33. doi: 10.1083/jcb.201211069.
PMID: 23439681

In vitro studies of actin filament and network dynamics.
Mullins RD, Hansen SD.
Curr Opin Cell Biol. 2013 Feb;25(1):6-13. doi: 10.1016/j.ceb.2012.11.007. Review.
PMID: 23267766

Graduate education. Interdisciplinary graduate training in teaching labs.
Vale RD, DeRisi J, Phillips R, Mullins RD, Waterman C, Mitchison TJ.
Science. 2012 Dec 21;338(6114):1542-3. doi: 10.1126/science.1216570.
PMID: 23258877

Differential remodeling of actin cytoskeleton architecture by profilin isoforms leads to distinct effects on cell migration and invasion.
Mouneimne G, Hansen SD, Selfors LM, Petrak L, Hickey MM, Gallegos LL, Simpson KJ, Lim J, Gertler FB, Hartwig JH, Mullins RD, Brugge JS.
Cancer Cell. 2012 Nov 13;22(5):615-30. doi: 10.1016/j.ccr.2012.09.027.
PMID: 23153535

The instability of stabilization.
Mullins RD.
Proc Natl Acad Sci U S A. 2012 Jul 3;109(27):10743-4. doi: 10.1073/pnas.1207980109.
PMID: 22733745

Actin binding to WH2 domains regulates nuclear import of the multifunctional actin regulator JMY.
Zuchero JB, Belin B, Mullins RD.
Mol Biol Cell. 2012 Mar;23(5):853-63. doi: 10.1091/mbc.E11-12-0992.
PMID: 22262458

Architecture and assembly of a divergent member of the ParM family of bacterial actin-like proteins.
Rivera CR, Kollman JM, Polka JK, Agard DA, Mullins RD.
J Biol Chem. 2011 Apr 22;286(16):14282-90. doi: 10.1074/jbc.M110.203828.
PMID: 21339292

VASP is a processive actin polymerase that requires monomeric actin for barbed end association.
Hansen SD, Mullins RD.
J Cell Biol. 2010 Nov 1;191(3):571-84. doi: 10.1083/jcb.201003014.
PMID: 21041447

Cytoskeletal mechanisms for breaking cellular symmetry.
Mullins RD.
Cold Spring Harb Perspect Biol. 2010 Jan;2(1):a003392. doi: 10.1101/cshperspect.a003392. Review.
PMID: 20182610

Cell mechanics and the cytoskeleton.
Fletcher DA, Mullins RD.
Nature. 2010 Jan 28;463(7280):485-92. doi: 10.1038/nature08908. Review.
PMID: 20110992

In silico reconstitution of actin-based symmetry breaking and motility.
Dayel MJ, Akin O, Landeryou M, Risca V, Mogilner A, Mullins RD.
PLoS Biol. 2009 Sep;7(9):e1000201. doi: 10.1371/journal.pbio.1000201.
PMID: 19771152

Structural polymorphism of the ParM filament and dynamic instability.
Galkin VE, Orlova A, Rivera C, Mullins RD, Egelman EH.
Structure. 2009 Sep 9;17(9):1253-64. doi: 10.1016/j.str.2009.07.008.
PMID: 19748346

The structure and assembly dynamics of plasmid actin AlfA imply a novel mechanism of DNA segregation.
Polka JK, Kollman JM, Agard DA, Mullins RD.
J Bacteriol. 2009 Oct;191(20):6219-30. doi: 10.1128/JB.00676-09.
PMID: 19666709

Bacterial Chromosome Segregation.
Mullins RD.
Annu Rev Cell Dev Biol. 2009 Jan 12.
PMID: 19575665

p53-cofactor JMY is a multifunctional actin nucleation factor.
Zuchero JB, Coutts AS, Quinlan ME, Thangue NB, Mullins RD.
Nat Cell Biol. 2009 Apr;11(4):451-9. doi: 10.1038/ncb1852.
PMID: 19287377

Phosphorylation of the Arp2/3 complex is necessary to nucleate actin filaments.
LeClaire LL 3rd, Baumgartner M, Iwasa JH, Mullins RD, Barber DL.
J Cell Biol. 2008 Aug 25;182(4):647-54. doi: 10.1083/jcb.200802145.
PMID: 18725535

Protein-nanocrystal conjugates support a single filament polymerization model in R1 plasmid segregation.
Choi CL, Claridge SA, Garner EC, Alivisatos AP, Mullins RD.
J Biol Chem. 2008 Oct 17;283(42):28081-6. doi: 10.1074/jbc.M803833200.
PMID: 18658133

The pathogen protein EspF(U) hijacks actin polymerization using mimicry and multivalency.
Sallee NA, Rivera GM, Dueber JE, Vasilescu D, Mullins RD, Mayer BJ, Lim WA.
Nature. 2008 Aug 21;454(7207):1005-8. doi: 10.1038/nature07170.
PMID: 18650806

Capping protein increases the rate of actin-based motility by promoting filament nucleation by the Arp2/3 complex.
Akin O, Mullins RD.
Cell. 2008 May 30;133(5):841-51. doi: 10.1016/j.cell.2008.04.011.
PMID: 18510928

Trapping fugitive filament formers.
Mullins RD.
Structure. 2008 May;16(5):661-3. doi: 10.1016/j.str.2008.04.006.
PMID: 18462670

In vivo visualization of type II plasmid segregation: bacterial actin filaments pushing plasmids.
Campbell CS, Mullins RD.
J Cell Biol. 2007 Dec 3;179(5):1059-66.
PMID: 18039937

Regulatory interactions between two actin nucleators, Spire and Cappuccino.
Quinlan ME, Hilgert S, Bedrossian A, Mullins RD, Kerkhoff E.
J Cell Biol. 2007 Oct 8;179(1):117-28.
PMID: 17923532

The structure of bacterial ParM filaments.
Orlova A, Garner EC, Galkin VE, Heuser J, Mullins RD, Egelman EH.
Nat Struct Mol Biol. 2007 Oct;14(10):921-6.
PMID: 17873883

Reconstitution of DNA segregation driven by assembly of a prokaryotic actin homolog.
Garner EC, Campbell CS, Weibel DB, Mullins RD.
Science. 2007 Mar 2;315(5816):1270-4.
PMID: 17332412

Spatial and temporal relationships between actin-filament nucleation, capping, and disassembly.
Iwasa JH, Mullins RD.
Curr Biol. 2007 Mar 6;17(5):395-406.
PMID: 17331727

Targeted wild-type and jerker espins reveal a novel, WH2-domain-dependent way to make actin bundles in cells.
Loomis PA, Kelly AE, Zheng L, Changyaleket B, Sekerková G, Mugnaini E, Ferreira A, Mullins RD, Bartles JR.
J Cell Sci. 2006 Apr 15;119(Pt 8):1655-65.
PMID: 16569662

Actin binding to the central domain of WASP/Scar proteins plays a critical role in the activation of the Arp2/3 complex.
Kelly AE, Kranitz H, Dötsch V, Mullins RD.
J Biol Chem. 2006 Apr 14;281(15):10589-97.
PMID: 16403731

Drosophila Spire is an actin nucleation factor.
Quinlan ME, Heuser JE, Kerkhoff E, Mullins RD.
Nature. 2005 Jan 27;433(7024):382-8.
PMID: 15674283

Dynamic instability in a DNA-segregating prokaryotic actin homolog.
Garner EC, Campbell CS, Mullins RD.
Science. 2004 Nov 5;306(5698):1021-5.
PMID: 15528442

Activation of Arp2/3 complex: addition of the first subunit of the new filament by a WASP protein triggers rapid ATP hydrolysis on Arp2.
Dayel MJ, Mullins RD.
PLoS Biol. 2004 Apr;2(4):E91.
PMID: 15094799

Cellular control of actin nucleation.
Welch MD, Mullins RD.
Annu Rev Cell Dev Biol. 2002;18:247-88. Review.
PMID: 12142287

Arp2/3 complex requires hydrolyzable ATP for nucleation of new actin filaments.
Dayel MJ, Holleran EA, Mullins RD.
Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):14871-6.
PMID: 11752435
Different WASP family proteins stimulate different Arp2/3 complex-dependent actin-nucleating activities.
Zalevsky J, Lempert L, Kranitz H, Mullins RD.
Curr Biol. 2001 Dec 11;11(24):1903-13.
PMID: 11747816

Calcium spiking activity and baseline calcium levels in ROS 17/2.8 cells exposed to extremely low frequency electromagnetic fields (ELF EMF).
Shahidain R, Mullins RD, Sisken JE.
Int J Radiat Biol. 2001 Feb;77(2):241-8.
PMID: 11236931

Actin dynamics.
Pollard TD, Blanchoin L, Mullins RD.
J Cell Sci. 2001 Jan;114(Pt 1):3-4.
PMID: 11112680

Interactions of ADF/cofilin, Arp2/3 complex, capping protein and profilin in remodeling of branched actin filament networks.
Blanchoin L, Pollard TD, Mullins RD.
Curr Biol. 2000 Oct 19;10(20):1273-82.
PMID: 11069108

Integration of multiple signals through cooperative regulation of the N-WASP-Arp2/3 complex.
Prehoda KE, Scott JA, Mullins RD, Lim WA.
Science. 2000 Oct 27;290(5492):801-6.
PMID: 11052943

Actin assembly mediated by Arp2/3 complex and WASP family proteins.
Mullins RD, Machesky LM.
Methods Enzymol. 2000;325:214-37.
PMID: 11036606

Activation of the Arp2/3 complex by the Listeria acta protein. Acta binds two actin monomers and three subunits of the Arp2/3 complex.
Zalevsky J, Grigorova I, Mullins RD.
J Biol Chem. 2001 Feb 2;276(5):3468-75.
PMID: 11029465

Molecular mechanisms controlling actin filament dynamics in nonmuscle cells.
Pollard TD, Blanchoin L, Mullins RD.
Annu Rev Biophys Biomol Struct. 2000;29:545-76. Review.
PMID: 10940259

Mechanisms underlying spontaneous calcium spiking in aequorin-loaded ROS 17/2.8 cells.
Mullins RD, Sisken JE.
Bioelectromagnetics. 2000 Jul;21(5):329-37.
PMID: 10899768

beta-arrestin-dependent endocytosis of proteinase-activated receptor 2 is required for intracellular targeting of activated ERK1/2.
DeFea KA, Zalevsky J, Thoma MS, Déry O, Mullins RD, Bunnett NW.
J Cell Biol. 2000 Mar 20;148(6):1267-81.
PMID: 10725339

How WASP-family proteins and the Arp2/3 complex convert intracellular signals into cytoskeletal structures.
Mullins RD.
Curr Opin Cell Biol. 2000 Feb;12(1):91-6. Review.
PMID: 10679362

Structure and function of the Arp2/3 complex.
Mullins RD, Pollard TD.
Curr Opin Struct Biol. 1999 Apr;9(2):244-9. Review.
PMID: 10322212

Rho-family GTPases require the Arp2/3 complex to stimulate actin polymerization in Acanthamoeba extracts.
Mullins RD, Pollard TD.
Curr Biol. 1999 Apr 22;9(8):405-15.
PMID: 10226024

Scar, a WASp-related protein, activates nucleation of actin filaments by the Arp2/3 complex.
Machesky LM, Mullins RD, Higgs HN, Kaiser DA, Blanchoin L, May RC, Hall ME, Pollard TD.
Proc Natl Acad Sci U S A. 1999 Mar 30;96(7):3739-44.
PMID: 10097107

Purification and assay of the Arp2/3 complex from Acanthamoeba castellanii.
Kelleher JF, Mullins RD, Pollard TD.
Methods Enzymol. 1998;298:42-51.
PMID: 9751870

The interaction of Arp2/3 complex with actin: nucleation, high affinity pointed end capping, and formation of branching networks of filaments.
Mullins RD, Heuser JA, Pollard TD.
Proc Natl Acad Sci U S A. 1998 May 26;95(11):6181-6.
PMID: 9600938

Arp2/3 complex from Acanthamoeba binds profilin and cross-links actin filaments.
Mullins RD, Kelleher JF, Xu J, Pollard TD.
Mol Biol Cell. 1998 Apr;9(4):841-52.
PMID: 9529382

Structure, subunit topology, and actin-binding activity of the Arp2/3 complex from Acanthamoeba.
Mullins RD, Stafford WF, Pollard TD.
J Cell Biol. 1997 Jan 27;136(2):331-43.
PMID: 9015304

Actin’ like actin?
Mullins RD, Kelleher JF, Pollard TD.
Trends Cell Biol. 1996 Jun;6(6):208-12.
PMID: 15157457

Serum- and bradykinin-induced calcium transients in familial Alzheimer’s fibroblasts.
McCoy KR, Mullins RD, Newcomb TG, Ng GM, Pavlínková G, Polinsky RJ, Nee LE, Sisken JE.
Neurobiol Aging. 1993 Sep-Oct;14(5):447-55.
PMID: 8247227

Altered calcium regulation in SV40-transformed Swiss 3T3 fibroblasts.
Newcomb TG, Mullins RD, Sisken JE.
Cell Calcium. 1993 Jul;14(7):539-49.
PMID: 8402837

Design and characterization of a system for exposure of cultured cells to extremely low frequency electric and magnetic fields over a wide range of field strengths.
Mullins RD, Sisken JE, Hejase HA, Sisken BF.
Bioelectromagnetics. 1993;14(2):173-86.
PMID: 8494558