SDS-PAGE
HOW DOES AN SDS PAGE GEL REALLY WORK? Dyche Mullins 2/1/2002 The system most people use for separating proteins by […]
protein purification
protein purification
Profilin Purification
Purification of recombinant profilin from E. coli RDM 4/30/03 Lysis buffer 10 mM Tris-HCl pH 8.0 1 mM EDTA 1
protein purification
Making Poly-L-proline Columns for Profilin Purification
Making a poly-L-proline Sepharose column for purification of profilin RDM (4/30/03) N.B. This protocol makes a lifetime supply of pLp
protein purification
Purification of α-actinin from Acanthamoeba
Dyche Mullins 11/98 1. Follow general protocol for amoeba prep using sucrose buffers. Elute protein from DEAE column with 0.0-0.6 M
protein purification
Purification of Capping Protein from Acanthamoeba
Dyche Mullins 11/98 1. Follow general protocol for amoeba prep using sucrose buffers. Elute protein from DEAE column with 0.0-1.0
protein purification
Pyrene Fluorescence Assay II
Pyrene fluorescence assay of actin assembly II Dyche Mullins, 9/13/99 Stock solutions: Buffer A (a.k.a. G buffer): 2 mM Tris
protein purification
Pyrene Fluorescence Assay
Pyrene Fluorescence Assay of Actin Assembly Dyche Mullins, 9/13/99 Stock solutions: Buffer A (a.k.a. G buffer): 2 mM Tris HCl,