Research Focus:
My research interests are in elucidating how bacteria impart spatial organization to their chromosomes and protiein components within their tiny volumes. I study this problem though microscopic and reductionist biochemical apporaches, and hope to develop tools to directly study the organization of these cells in real time.

Background:

Currently I am a Helen Hay Whiney Fellow working in the series of labs at Harvard and MIT. My primary lab homes are with Tim Mitchison, Alice Ting, and Xiowei Zhaung.

I attained my PhD in biochemistry at UCSF in San Francisco, studying in the lab of Dyche Mullins, where I reconstituted DNA segregation by the par operon of the low copy R1 plasmid.

I earned a BS in Biochemistry at WSU under the supervision of Keith Dunker and Zoran Obradovic , where I worked upon development and genomic applications of predictors of protein disorder from primary sequence data. Both have left WSU for greener pastures. I was a bioinformaticist in this former life,.   Although I am primary a wet lab researcher now now, I still waste incredible amounts of time coding random scripts and projects, and breaking my linux box almost daily.

In my non-lab time I enjoy coding random applications to analyze the rental markets of various cities, and on the physical side, I enjoy building interactive fire art incorporating a mix of colored fire with childrens toys withthe group FalseProfitLabs

Software Projects I am (or was) involved in:
CraigStatsSF - An ongoing spatial database of apartment listings in San Francisco from Craigslist. Complete with hetmaps and spatial overlays.
PONDR - predictor of disordered  regions from amino acid sequence
dbPONDR - Large scale genomic analysis of disorder in respect to sequence, structure, motifs, and property/predictor I could get my hands on.
phplabware -  point and click web based database frontend for lab administration or whatever you want.

Random Press from hobbies:
FalseProfitLabs - Fire Art done by a collection of engineers, computer kids, and biologists.
Writeups on CraigstatsSF from Consumerist and BoingBoing.
SF Chronicle Article on the rent spike in San Francisco.

Publications:

Garner EC, Campbell CS, Weibel DB, Mullins RD. Reconstitution of DNA segregation driven by assembly of a prokaryotic actin homolog. Science. 2007 Mar 2;315(5816):1270-4.
Abstract | Full Text

Garner EC, Campbell CS, Mullins RD. Dynamic instability in a DNA-segregating prokaryotic actin homolog. Science. 2004 Nov 5;306(5698):1021-5.
Abstract | Full Text

Protein-nanocrystal conjugates support a single filament polymerization model in R1 plasmid segregation.
Choi CL, Claridge SA, Garner EC, Alivisatos AP, Mullins RD. J Biol Chem. 2008 Oct 17;283(42):28081-6.

The structure of bacterial ParM filaments.
Nat Struct Mol Biol. 2007 Oct;14(10):921-6. Epub 2007 Sep 16. Orlova A, Garner EC, Galkin VE, Heuser J, Mullins RD, Egelman EH.

Predicting Binding Regions within Disordered Proteins.
Garner E, Romero P, Dunker AK, Brown C, Obradovic Z.
Genome Informatics Workshop 1999; 10:41-50

Predicting Disordered Regions from Amino Acid Sequence: Common Themes Despite Differing Structural Characterizations
Garner E, Cannon P, Romero P, Obradovic Z, Dunker AK.
Genome Informatics 1998; 9: 201-214

Clusterin, a binding protein with a molten globule-like region.
Bailey RW, Dunker AK, Brown CJ, Garner EC, Griswold MD.
Biochemistry. 2001 Oct 2;40(39):11828-40.

Identification of intrinsic order and disorder in the DNA repair protein XPA.
Iakoucheva LM, Kimzey AL, Masselon CD, Bruce JE, Garner EC, Brown CJ, Dunker AK, Smith RD, Ackerman EJ.
Protein Sci. 2001 Mar;10(3):560-71.

Sequence complexity of disordered protein.
Romero P, Obradovic Z, Li X, Garner E, Brown C, Dunker AK.
Proteins. 2001 Jan 1;42(1):38-48.

The power to detect recombination using the coalescent.
Brown CJ, Garner EC, Keith Dunker A, Joyce P.
Mol Biol Evol. 2001 Jul;18(7):1421-4.

Intrinsically disordered protein.
Dunker AK, Lawson JD, Brown CJ, Williams RM, Romero P, Oh JS, Oldfield CJ, Campen AM, Ratliff CM, Hipps KW, Ausio J, Nissen MS, Reeves R, Kang C, Kissinger CR, Bailey RW, Griswold MD, Chiu W, Garner EC, Obradovic Z.
  J Mol Graph Model. 2001;19(1):26-59.

The protein non-folding problem: amino acid determinants of intrinsic order and disorder.
Williams RM, Obradovic Z, Mathura V, Braun W, Garner EC, Young J, Takayama S, Brown CJ, Dunker AK.
Pac Symp Biocomput. 2001; 4:89-100.

Intrinsic Protein Disorder in Complete Genomes.
Dunker AK, Obradovic Z, Romero P, Garner EC.
Genome Informatics 2000; 11: 161-171.

Comparing Predictors of Disordered Protein.
Li X, Obradovic Z, Brown CJ, Garner EC, Dunker AK.
Genome Informatics 2000; 11: 172-184.

Isolation and characterization of cDNAs expressed in the early stages of flavonol-induced pollen germination in petunia.
Guyon VN, Astwood JD, Garner EC, Dunker AK, Taylor LP. Plant Physiol. 2000; Jun 123(2):699-710.

The Sequence Attribute Method for Determining Relationships Between Sequence and Protein Disorder.
Xie Q, Arnold GE, Romero P, Obradovic Z, Garner E, Dunker AK.
Genome Informatics. 1998; 9:201-213

Thousands of Proteins Likely to Have Long Disordered Regions.
P. Romero, Z. Obradovic, C.R. Kissinger, J.E. Villafranca, E. Garner, S. Guilliot and A.K. Dunker
Pacific Symposium on Biocomputing 1998; 3:435-446.

Protein Disorder and the Evolution of Molecular Recognition: Theory, Predictions, and Observations.
A.K. Dunker, E. Garner, S. Guilliot, P. Romero, K. Albrecht, J. Hart, Z. Obradovic, C. Kissinger and E. Villafranca.
Pacific Symposium on Biocomputing 1998; 3:471-482.